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Écouvillon nasopharyngeal

» Écouvillon nasopharyngeal

Comment prélever des spécimens pour améliorer la précision?

Écouvillon nasopharyngeal, écouvillon oropharyngéal
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The virus sampling tube is used for the collection and transportation of new coronacirus, influenza virus, grippe aviaire (such as H7N9), hand, foot and mouth virus, measles and other virus specimens, as well as mycoplasma, ureaplasma, and chlamydia specimens.

Viruses and related samples; stored and transported within 48 hours under refrigerated conditions (2~8°C). Long-term storage of viruses and related samples in -80°C or liquid nitrogen environment.

The virus preservation liquid of the virus sampling tube is mainly composed of Hank’s liquid base, gentamicin, fungal antibiotics, biological buffer and amino acids. It can maintain the activity of the virus in a wide temperature range, reduce the speed of virus decomposition, and increase the positive rate of virus isolation.

Will clinicians have such troubles. The patient is highly suspected of new coronavirus pneumonia, but the two swab nucleic acid tests were negative. What should I do? Or maybe it was two swab tests that were negative, but many people in contact with him were diagnosed. Is he true negative or false negative? Comment prélever des spécimens pour améliorer la précision?

Specimen collection site:

Many experts have summarized the experience from practical work that the positive rate of 2019-nCOV nucleic acid test in nasopharyngeal swab specimens is higher than that of oropharyngeal swabs, and the positive rate of sputum and alveolar lavage samples collected from the lower respiratory tract is higher than that of the upper respiratory tract. , Nasopharyngeal swab specimens, but the lower respiratory tract sputum, alveolar lavage fluid specimens are difficult to collect, and it is easy to cause the patient to spray and cause the operator to have a high risk of infection. Généralement, it is not recommended to use (tracheotomy, ventilator rescue) Available to patients). The fifth edition of the recently releasedPneumonia Diagnosis and Treatment Plan for Novel Coronavirus Infectionhas updated the specimen collectionpharyngeal swab” À “nasopharyngeal swab”. In order to increase the positive rate of testing, it is recommended to collect samples from multiple parts of the same patient and combine them for testing. Par exemple,, the oropharyngeal swab and nasopharyngeal swab are used to collect specimens at the same time, and then they are placed in the same collection tube for examination. For suspected patients with gastrointestinal symptoms, feces and anal swabs are collected for testing at the same time.

Specimen collection method: The most recently used for nucleic acid detection samples are oropharyngeal swabs. When collecting, let the patient open his mouth and use the sampling swab to scrape the virus-carrying specimens from the pharynx. If the medical staff usually don’t take a lot of throat swabs, and the patient’s response when scraping the sample is relatively large, it often results in the wrong location of the sample, or insufficient strength and time, and no virus-carrying specimens or scraping strips are scraped. The small amount of virus specimens eventually led to a negative test result.

Specimen transportation and storage conditions:

2019-nCOV is an RNA virus, which is easily degraded by RNase released by exogenous or cell destruction, which affects the final detection efficiency. Strictly speaking, specimens should be sent to laboratories (CDC, hospital laboratory, third-party laboratories) in time after collection, and testing should be completed as soon as possible. If the specimen is placed in room temperature for too long (several hours, ten hours, twenty hours or more), it may also be the cause of the false negative result of the final test. Recently, some people have proposed the use of anti-virus nucleic acid degradation sample sampling tubes, but at present such products are small in number, high in cost, and practical application effects need to be further evaluated. It is recommended that the specimens be sent for inspection in time after collection, and tested in time (4 heures). If for some reason, it cannot be sent for inspection or tested in time after collection, it should be stored at 4°C (24 heures). For specimens that cannot be detected within 24 heures, it is recommended to store them below -70°C and avoid repeated freezing and thawing.

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